| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sabol, Mackinzie I (7)
|
MRRA |
45.74 |
43.54 |
-2.20 |
| 2 |
Brennan, Jane E (7)
|
MRRA |
44.33 |
46.16 |
1.83 |
| 3 |
Babyak, Eliza G (7)
|
MREAST |
46.27 |
47.17 |
0.90 |
| 4 |
Benerofe, Noa S (7)
|
MREAST |
53.17 |
47.51 |
-5.66 |
| 5 |
Nam, Lauren (7)
|
MRLIAC |
48.42 |
49.01 |
0.59 |
| 6 |
Euyoque, Nicole (7)
|
MRYMID |
50.57 |
49.03 |
-1.54 |
| 7 |
Valerio, Sara A (7)
|
MRYMID |
49.66 |
50.04 |
0.38 |
| 8 |
Costabile, Kylie (7)
|
MRCFB |
52.48 |
50.23 |
-2.25 |
| 9 |
Bartolacci, Abigail J (7)
|
MRRA |
1:00.45 |
54.27 |
-6.18 |
| 10 |
McCann, Evelyn E (6)
|
MRYMID |
|
57.37 |
- |
| 11 |
Cook, Keiran R (7)
|
MRLIAC |
1:08.01 |
57.87 |
-10.14 |
| 12 |
Burriss, Leah S (7)
|
MREAST |
|
59.52 |
- |
| 13 |
Gregersen, Leah (6)
|
MRRA |
1:00.50 |
1:02.18 |
1.68 |
| 14 |
Benjamin, Mia (7)
|
MRRA |
1:06.54 |
1:09.21 |
2.67 |
|
Martinez-Jimenez, Alba (6)
|
MRYMID |
1:05.00 |
NS |
- |